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Monoclonal Antibody Services> | … Mouse monoclonal anti-CC1, Calbiochem, Cat# op-80, RRID: AB_2057371. Rabbit polyclonal anti-CNPase, Genscript, Cat# A01308-40, RRID:AB_1575797. Mouse monoclonal anti-GFAP, Santa cruz, Cat# sc-65,343, RRID:AB_783553 … | Get A Quote |
PDK1 (3-Phosphoinositide dependent protein kinase-1) is a member in the PI3K (phosphatidylinositol 3 kinase) pathway and is implicated in neurodevelopmental disease with microcephaly. Although the role of PDK1 in neurogenesis has been broadly studied, it remains unknown how PDK1 may regulate oligogenesis in the central nervous system (CNS). To address this question, we generated oligodendrocyte (OL) lineage cells specific PDK1 conditional knockout (cKO) mice. We find that PDK1 cKOs display abnormal white matter (WM), massive loss of mature OLs and severe defect in myelination in the CNS. In contrast, these mutants exhibit normal neuronal development and unchanged apoptosis in the CNS. We demonstrate that deleti... More